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cd40l cd154  (Miltenyi Biotec)


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    Miltenyi Biotec cd40l cd154
    Cd40l Cd154, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd40l/CD154+Antibody%2C+anti-human%2C+REAfinity/pm41963361-193-19-22
    Average 94 stars, based on 44 article reviews
    cd40l cd154 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Citrullinated Autoantigen-Specific T and B Lymphocytes in Rheumatoid Arthritis: Focus on Follicular T Helper Cells and Expansion by Coculture.
    Article Snippet: Just before B cell addition, BSupps were washed twice and fresh X-Vivo15 medium (Lonza) was added. .. As controls to cocultures, B cells were cultured solely without feeder cells in basic X-Vivo15 medium or in medium containing B cell stimulants, including CD40L, IL-4, and anti-IgM (X-Vivo15 with 5 μg/mL of human CD40L, Miltenyi Biotech; 25 ng/mL of human IL-4, Miltenyi Biotech; 5 μg/mL of anti-human IgM, Johnson & Johnson). ..

    Article Title: Inhibition of A2AR alleviates adenosine-mediated suppression of plasma cell differentiation
    Article Snippet: .. Step 1 (day 0-4), expansion phase: after purification, memory B cells were seeded at 7.5 x 10 4 to 1.5 x 10 5 cells/well and cultured in complete IMDM medium supplemented with 20 U/ml IL-2 (Miltenyi), 50 ng/ml IL-10 (Peprotech), 10 ng/ml IL-15 (Peprotech), 10 μg/ml CpG (Invivogen) and 50 ng/ml CD40L (Miltenyi). ..

    Isolation:

    Article Title: CD8 + T cell-derived CD40L mediates noncanonical cytotoxicity in CD40-expressing cancer cells.
    Article Snippet: .. For the transfer in RAG1−/−, CD8+, and CD4+ T cells from C57BL/6 (or CD45.1) or CD40L−/− mice, T cells from splenocytes were isolated by magnetic microbeads (Miltenyi Biotec). ..

    Article Title: Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency.
    Article Snippet: .. Subsequently, to analyze CD40L binding to CD40 on B cell surface, peripheral memory B cells were isolated from the blood of P1 using a memory B cell selection kit (Miltenyi, 130-093-546) and co-incubated with the supernatant of C. albicans-platelets co-culture for 30 min at 37 °C. .. The bound CD40L on the B cell surface was quantified by flow cytometry using a FITC-labeled anti-human CD40L antibody (Biolegend, 310804).

    Article Title: Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency
    Article Snippet: .. Subsequently, to analyze CD40L binding to CD40 on B cell surfaces, peripheral memory B cells were isolated from the blood of P1 using a memory B cell selection kit (Miltenyi, 130-093-546) and co-incubated with the supernatant of C . albicans –platelets co-culture for 30 minutes at 37°C. .. The bound CD40L on the B cell surface was quantified by flow cytometry using a FITC-labeled anti–human CD40L antibody (BioLegend, 310804).

    Article Title: Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency
    Article Snippet: .. Subsequently, to analyze CD40L binding to CD40 on B cell surface, peripheral memory B cells were isolated from the blood of P1 using a memory B cell selection kit (Miltenyi, 130-093-546) and co-incubated with the supernatant of C. albicans-platelets co-culture for 30 min at 37 °C. .. The bound CD40L on the B cell surface was quantified by flow cytometry using a FITC-labeled anti-human CD40L antibody (Biolegend, 310804).

    Binding Assay:

    Article Title: Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency.
    Article Snippet: .. Subsequently, to analyze CD40L binding to CD40 on B cell surface, peripheral memory B cells were isolated from the blood of P1 using a memory B cell selection kit (Miltenyi, 130-093-546) and co-incubated with the supernatant of C. albicans-platelets co-culture for 30 min at 37 °C. .. The bound CD40L on the B cell surface was quantified by flow cytometry using a FITC-labeled anti-human CD40L antibody (Biolegend, 310804).

    Article Title: Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency
    Article Snippet: .. Subsequently, to analyze CD40L binding to CD40 on B cell surfaces, peripheral memory B cells were isolated from the blood of P1 using a memory B cell selection kit (Miltenyi, 130-093-546) and co-incubated with the supernatant of C . albicans –platelets co-culture for 30 minutes at 37°C. .. The bound CD40L on the B cell surface was quantified by flow cytometry using a FITC-labeled anti–human CD40L antibody (BioLegend, 310804).

    Article Title: Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency
    Article Snippet: .. Subsequently, to analyze CD40L binding to CD40 on B cell surface, peripheral memory B cells were isolated from the blood of P1 using a memory B cell selection kit (Miltenyi, 130-093-546) and co-incubated with the supernatant of C. albicans-platelets co-culture for 30 min at 37 °C. .. The bound CD40L on the B cell surface was quantified by flow cytometry using a FITC-labeled anti-human CD40L antibody (Biolegend, 310804).

    Selection:

    Article Title: Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency.
    Article Snippet: .. Subsequently, to analyze CD40L binding to CD40 on B cell surface, peripheral memory B cells were isolated from the blood of P1 using a memory B cell selection kit (Miltenyi, 130-093-546) and co-incubated with the supernatant of C. albicans-platelets co-culture for 30 min at 37 °C. .. The bound CD40L on the B cell surface was quantified by flow cytometry using a FITC-labeled anti-human CD40L antibody (Biolegend, 310804).

    Article Title: Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency
    Article Snippet: .. Subsequently, to analyze CD40L binding to CD40 on B cell surfaces, peripheral memory B cells were isolated from the blood of P1 using a memory B cell selection kit (Miltenyi, 130-093-546) and co-incubated with the supernatant of C . albicans –platelets co-culture for 30 minutes at 37°C. .. The bound CD40L on the B cell surface was quantified by flow cytometry using a FITC-labeled anti–human CD40L antibody (BioLegend, 310804).

    Article Title: Combined FXIII-C3 autoantibodies elicit bleeding and complement dysfunction in autoimmune FXIII deficiency
    Article Snippet: .. Subsequently, to analyze CD40L binding to CD40 on B cell surface, peripheral memory B cells were isolated from the blood of P1 using a memory B cell selection kit (Miltenyi, 130-093-546) and co-incubated with the supernatant of C. albicans-platelets co-culture for 30 min at 37 °C. .. The bound CD40L on the B cell surface was quantified by flow cytometry using a FITC-labeled anti-human CD40L antibody (Biolegend, 310804).

    Staining:

    Article Title: Enhanced anti-tumor activity by zinc finger repressor-driven epigenetic silencing of immune checkpoints and TGFBR2 in CAR-T cells and TILs
    Article Snippet: Human primary cells immunophenotyping For cellular immunophenotyping, T cells were stained with conjugated monoclonal antibodies (mAb) targeting HA (RRID:AB_2784359), PD-1 (RRID:AB_2752070), TIGIT (RRID:AB_2751336), TIM-3 (RRID:AB_RRID:AB_2657763), CD271/NGFR (RRID:AB_2725863) (Miltenyi Biotec), LAG-3 (RRID:AB_2632656), and TGFBR2 (RRID: AB_2876726, BioLegend, Amsterdam, the Netherlands). .. For TIL characterization, expanded cells were stained with fluorophore-labeled antibodies against CD3 (RRID:AB_493741), CD4 (RRID:AB_2563314), CD8 (RRID:AB_1967149), CD271/NGFR (RRID:AB_2282828), PD-1 (RRID:AB_2563658), TIGIT (RRID:AB_2632929), TIM-3 (RRID: AB_11218598), LAG-3 (RRID:AB_2632656), TGFBR2 (RRID: AB_2876726), CD45RA (RRID:AB_2563816), CCR7 (RRID:AB_ 2563640), CD183/CXCR3 (RRID:AB_11219383), CD25 (RRID:AB_ 314280), CD28 (RRID:AB_2800752), KLRG1 (RRID:AB_2566594), CD62L (RRID:AB_2562130), CD127 (RRID:AB_10720185), FoxP3 (RRID:AB_430887), CD40L (RRID:AB_2562721), CD107a (RRID: AB_2563851, BioLegend), TNF-α (RRID:AB_2751384, Miltenyi Biotec), and IFN-γ (RRID:AB_396760, BD Biosciences, Heidelberg, Germany). .. After surface staining, FoxP3 was assessed after fixation and permeabilization using the BD Pharmingen Human FoxP3 Buffer Set (BD Biosciences).

    Article Title: Enhanced anti-tumor activity by Zinc Finger Repressor-driven epigenetic silencing of immune checkpoints and TGFBR2 in CAR-T cells and TILs
    Article Snippet: For cellular immunophenotyping, T cells were stained with conjugated monoclonal antibodies (mAb) targeting HA, PD-1, TIGIT, TIM-3, CD271/NGFR (Miltenyi Biotec), LAG-3 and TGFBR2 (Biolegend, Amsterdam, Netherlands). .. For TILs characterization, expanded cells were stained with fluorophore-labeled antibodies against CD3, CD4, CD8, CD271/NGFR, PD-1, TIGIT, TIM-3, LAG-3, TGFBR2, CD45RA, CCR7, CD183/CXCR3, CD25, CD28, KLRG1, CD62L, CD127, FoxP3, CD40L, CD107a (Biolegend), TNFα (Miltenyi Biotec) and IFNγ (BD Biosciences, Heidelberg, Germany). .. After surface staining, FoxP3 was assessed after fixation and permeabilization using the BD PharmingenTM Human FoxP3 Buffer Set (BD Biosciences).

    Purification:

    Article Title: Inhibition of A2AR alleviates adenosine-mediated suppression of plasma cell differentiation
    Article Snippet: .. Step 1 (day 0-4), expansion phase: after purification, memory B cells were seeded at 7.5 x 10 4 to 1.5 x 10 5 cells/well and cultured in complete IMDM medium supplemented with 20 U/ml IL-2 (Miltenyi), 50 ng/ml IL-10 (Peprotech), 10 ng/ml IL-15 (Peprotech), 10 μg/ml CpG (Invivogen) and 50 ng/ml CD40L (Miltenyi). ..



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    (A) Experimental schematic for B-C . (B) Plot shows the percentage of GC B cells of B220+ cells in mice pre-infused with 3BNC117 IgG2a in concert <t>with</t> <t>anti-CD40L</t> (maroon, for B-C ) or isotype control (pink, for B-C ). (C) Plot shows the percentage of PBs in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L or isotype control. (D) Experimental schematic for E-J . (E) Plot shows the percentage of GC B cells of B220+ cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a (+Ab, pink, for E-J ) or no antibody (control, -Ab, grey, for E-J ). (F) Plot shows the number of GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (G) Plot shows the percentage of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (H) Plot shows the number of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (I) Plot shows the percentage of TM4-Core+ cells of GC B cells in Cr2 KO mice pre- infused with 3BNC117 IgG2a or control. (J) Plot shows the number of TM4-Core+ GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. Data is displayed as mean ± SD. Each point represents a single mouse from at least two independent experiments. Statistical significance for B-C was determined using two-tailed unpaired t tests with Welch’s correction. Statistical significance for E-J was determined using two-tailed Mann-Whitney U tests. ns denotes non-significant, * denotes p ≤ 0.05, ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.
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    (A) Experimental schematic for B-C . (B) Plot shows the percentage of GC B cells of B220+ cells in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L (maroon, for B-C ) or isotype control (pink, for B-C ). (C) Plot shows the percentage of PBs in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L or isotype control. (D) Experimental schematic for E-J . (E) Plot shows the percentage of GC B cells of B220+ cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a (+Ab, pink, for E-J ) or no antibody (control, -Ab, grey, for E-J ). (F) Plot shows the number of GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (G) Plot shows the percentage of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (H) Plot shows the number of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (I) Plot shows the percentage of TM4-Core+ cells of GC B cells in Cr2 KO mice pre- infused with 3BNC117 IgG2a or control. (J) Plot shows the number of TM4-Core+ GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. Data is displayed as mean ± SD. Each point represents a single mouse from at least two independent experiments. Statistical significance for B-C was determined using two-tailed unpaired t tests with Welch’s correction. Statistical significance for E-J was determined using two-tailed Mann-Whitney U tests. ns denotes non-significant, * denotes p ≤ 0.05, ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.

    Journal: bioRxiv

    Article Title: Fc receptor dependent and independent mechanisms of antibody-mediated enhancement of immune responses

    doi: 10.64898/2026.03.27.714269

    Figure Lengend Snippet: (A) Experimental schematic for B-C . (B) Plot shows the percentage of GC B cells of B220+ cells in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L (maroon, for B-C ) or isotype control (pink, for B-C ). (C) Plot shows the percentage of PBs in mice pre-infused with 3BNC117 IgG2a in concert with anti-CD40L or isotype control. (D) Experimental schematic for E-J . (E) Plot shows the percentage of GC B cells of B220+ cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a (+Ab, pink, for E-J ) or no antibody (control, -Ab, grey, for E-J ). (F) Plot shows the number of GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (G) Plot shows the percentage of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (H) Plot shows the number of PBs in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. (I) Plot shows the percentage of TM4-Core+ cells of GC B cells in Cr2 KO mice pre- infused with 3BNC117 IgG2a or control. (J) Plot shows the number of TM4-Core+ GC B cells in Cr2 KO mice pre-infused with 3BNC117 IgG2a or control. Data is displayed as mean ± SD. Each point represents a single mouse from at least two independent experiments. Statistical significance for B-C was determined using two-tailed unpaired t tests with Welch’s correction. Statistical significance for E-J was determined using two-tailed Mann-Whitney U tests. ns denotes non-significant, * denotes p ≤ 0.05, ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.

    Article Snippet: Anti-CD40L (Clone: MR1, BioXCell BE0017-1) and the armenian hamster isotype control (BioXCell BE0091) were purchased from BioXCell.

    Techniques: Control, Two Tailed Test, MANN-WHITNEY